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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Oncol Rep. 2010 Apr;23(4):1109–1117. doi: 10.3892/or_00000739

Figure 6.

Figure 6

WT1-transfected MCF7L cells exhibit estrogen-independent and anti-estrogen insensitive growth (A). Growth rate of MCF7L cells transfected with the empty expression vector (MCF7/V) and clonal cell lines from MCF7L transfected with WT1 expression vector (MCF7/WT1-2, -3 and -4). Cells maintained in normal medium containing 5% FCS were seeded at 1×104 cells per well in 6-well plates and counted every other day. Data are shown as means ± SE from three separate experiments (B). Cells were seeded at 1×104 cells per 60-mm dishes, incubated in medium containing DMSO (V), 1 nM of 17β-estradiol (E2), 1 μM 4OH-TAM (4OH) or 1 μM ICI 182,780 (ICI) and counted at day 12. Column, means of three independent experiments; bars, SE. a–c, P<0.01 vs. MCF7/V cells; d–f, P<0.01 vs. MCF7/V cells.