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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Int J Oncol. 2010 Apr;36(4):809–816. doi: 10.3892/ijo_00000557

Figure 1. uPA and uPAR are knocked down in a radiation-inducible manner.

Figure 1

(A) IOMM Lee cells were transfected with pCArG-U2 and 24 h later, treated with 5 and 10 Gy. Non-transfected cells were maintained as controls. Cell lysates were collected and equal amounts of proteins were subjected to immunoblotting with uPA and uPAR antibodies. (B) Densitometric analysis of uPA and uPAR western blots. (C) IOMM Lee cells were transfected with pCArG-U2 and irradiated with 10 Gy. 48 h after transfection, total RNA was extracted from the cells as per standard protocols. Reverse transcription PCR analysis was performed using primers specific for uPA, uPAR and GAPDH. (D) Densitometric analysis of relative transcript levels of uPA and uPAR. Results from three independent experiments are shown as means ± S.D (p<0.05).