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. Author manuscript; available in PMC: 2011 Mar 2.
Published in final edited form as: Mol Cancer Ther. 2010 Mar 2;9(3):523–534. doi: 10.1158/1535-7163.MCT-09-0845

Figure 5. 2-ME2 restores E-cadherin protein in shRNA-transfected Bic-1 cells.

Figure 5

A. Transfected Bic-1 cells were treated with 5μM of 2-ME2 for 24 h or left untreated. Equal amounts of cell lysates (100μg/lane) were analyzed by Western blotting with an antibody against E-cadherin. Quantification of each band was performed by densitometry analysis software. * p<0.005 vs mismatched controls. ** p<0.01vs shRNA alone transfected cells.

B. E-cadherin and β-catenin shRNA transfected Bic-1 cells were exposed to 5 μM of 2-ME2 for 24 h, and β-catenin (left panel) and E-cadherin (right panel) mRNA expressions were determined by northern blotting using nonradioactive DIG-labeled probe. GAPDH is used as loading control.