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. 2010 Jan 19;102(4):765–773. doi: 10.1038/sj.bjc.6605515

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Copyright © 2010 Cancer Research UK

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The decrease in COX2 protein expression under NS398 treatment. Dose-dependent inhibition of COX2 protein expression was observed under NS398 treatment. Strong granular and/or diffuse cytoplasmatic immunostaining was detected in COX2-positive cells. (A) COX2 protein expression in untreated control HT29 cells. (B) COX2 protein expression in HT29 cells treated with 100 μM NS398 ( × 10 magnification, haematoxylin co-staining). (C) COX2 protein expression detected by western blot analysis. The diagram shows the band intensities determined by Molecular Imaging Software version 4.0.