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. 2010 Jan 19;102(4):738–747. doi: 10.1038/sj.bjc.6605517

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Copyright © 2010 Cancer Research UK

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E2 upregulates Shh expression through ERα activation in ERα-positive gastric cancer cells. (A) Gli1 mRNA in KATOIII, NCI-N87 and MK-1 cells treated with the indicated reagents for 8 h was examined by real-time RT-PCR. (B) Shh mRNA in KATOIII, NCI-N87 and MK-1 cells treated with the indicated reagents for 8 h was examined by real-time RT-PCR. (C) After transfection with ERα siRNA or control siRNA, KATOIII and NCI-N87 cells were treated with E2 for 8 h and then Shh mRNA was examined by real-time RT–PCR. Results are expressed as mean±s.d. ^*P<0.05.