Figure 2.

Trapping of a gene stimulatory for cell migration. (A) The indicated cells (5 × 10⁴) grown for 72 h with or without tetracycline (2 μg ml⁻¹) were added to the upper chamber of transwell inserts. The lower chamber contained 10% FBS. After 6 h, cells migrating to the lower surface of the membrane were stained, and the number of cells was counted in five fields and averaged (±s.e.). Data are representative of at least three experiments. (B) The indicated cells were treated with or without 2 μg ml⁻¹ tetracycline for 72 h, RNA was extracted and subjected to RT–PCR (35 cycles) using primers specific for CLCN4 and actin.