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. 2010 Jan 19;102(4):774–782. doi: 10.1038/sj.bjc.6605536

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Copyright © 2010 Cancer Research UK

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Reduced invasiveness of null CLCN4 fibroblasts. These assays were as described for Figure 5, but with the following exceptions. The indicated cells (20 000) were seeded in triplicate on Matrigel-coated porous filters (8 μm) and, after 22 h, were assayed and quantified for invasion. Data are shown as the average percentage area of the filter occupied with nuclei±s.d. values of at least four independent fields. Nuclei are marked with white arrows. Statistical analysis was performed using a Mann–Whitney U-Test.