Runx2 regulation and miRNA expression during adipocyte differentiation. (A–G): C3H10T1/2 cells were induced with adipocyte differentiation medium for 3 days and then harvested for examination of Runx2 expression by western blotting (A) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays of Runx2 (B) or miRNA expression as indicated. (H) RT-PCR results showed that miR-204/211 were expressed in C3H10T1/2, C2C12, ST2, and BMSCs. (I) hMSCs were treated with adipocyte differentiation medium for 8 days and harvested for miR-204/211 qRT-PCR assays. *, p < .05, unpaired Student’s t-test, n = 3. Abbreviations: hMSCs, human mesenchymal stem cells.