Table 1.

Biochemical properties of wild-type and mutated forms of CcGlgP.

	PLP	activity
	[μM/μM protein]	[U/mg]	kcat [s-1]
wild-type	0.7	31	47
T28A	0.6	29	44
K31A	0.6	29	44
S174A	0.7	29	44
R141A	0.7	27	40

Activities were measured at 30°C at pH 7.0 in a continuous coupled enzyme assay in the presence of 50 mM P_i and 24 g/l maltodextrin (Agenamalt 19DE). The k_cat value was calculated from the specific activity (U/mg) assuming a molecular mass of 90.6 kDa for the enzyme subunit. PLP values have S.D. of < 20% (N = 3) while activity values have S.D. of < 10% (N = 4).