Table 2.

Comparison of half-life times (t_1/2) of recombinant wild-type CcGlgP and site-directed enzyme variants in thermal denaturation experiments and in the presence of 0.4 M imidazole at 30°C

t (1/2) [min]	inactivation at 45°C			inactivation by imidazole at 30°C
	no Pi	10.0 mM Pi	100 mM Pi	no Pi	5.0 mM Pi
wild-type	2.6	2.8 × 102	3.5 × 103	22	160
T28A	0.8	2.2	3.3 × 102	1.9	5.6
K31A	5.3	1.7 × 102	3.5 × 103	10	25
S174A	2.8	33	9.9 × 102	14	58
R141A	2.9	7.5	1.4 × 103	6.4	27

The experiments were carried out in 50 mM triethanolamine buffer, pH 7.0. Values from linear regression analysis have S.D. of < 10%. Representative data were used for calculation of t_1/2.