Table 1.

Intra-assay and inter-assay of the TaqMan™ FQ-PCR assay

Variation	Copies of standard	Crossing point
		Mean	SDa	CV (%)b
Intra-assay	1.00E+08	19.73	0.15	0.77
	1.00E+07	23.10	0.20	0.87
	1.00E+06	26.37	0.29	1.09
	1.00E+05	29.63	0.21	0.70
	1.00E+04	33.07	0.31	0.92
	1.00E+03	36.33	0.31	0.84
	1.00E+02	39.50	0.17	0.44
	1.00E+01	42.67	0.32	0.75
Inter-assay	1.00E+08	19.70	0.28	1.41
	1.00E+07	23.09	0.47	2.03
	1.00E+06	26.31	0.32	1.23
	1.00E+05	29.59	0.42	1.42
	1.00E+04	33.04	0.34	1.03
	1.00E+03	36.35	0.41	1.14
	1.00E+02	39.52	0.43	1.10
	1.00E+01	42.70	0.41	0.97

Repeatability and reproducibility (R & R) of the TaqMan™ FQ-PCR assay.

^a Standard deviation.

^b Coefficient of variation.