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Oligonucleotide sequences of primers and probe used in AHV-1 FQ-PCR detection
| Name | Type | Sequences (5' to 3') | Length (nt) |
Amplicon size (bp) |
|
|---|---|---|---|---|---|
| P1 | Forward | CGGAATTCCAAAACGCCGCACAGATGAC | 28 | 1296 | |
| P2 | Reverse | CCCTCGAGGTATTCAAATAATATTGTCTGC | 30 | ||
| P3 | Forward | GAAGGACGGAATGGTGGAAG | 20 | 78 | |
| P4 | Reverse | AGCGGGTAACGAGATCTAATATTGA | 25 | ||
| P | Probe | FAM-CCAATGCATCGATCATCCCGGAA-TAMRA | 23 | ||
The underlined sequences of P1 and P2 are EcoR I and Xho I restriction sites respectively
