Table 1.
Interdomain hydrogen bonding interactions and corresponding distances (in Å) among A1 (1-330), A2 (381-740) and A3(1669-2019) domains of the zymogenic and activated forms of fVIII.
| fVIII zymogen | Activated fVIII | ||||
|---|---|---|---|---|---|
| A1:A2 interactions | |||||
| R240 (NH1) | L491 (O) | 3.1 | R240 (NH1) | L491 (O) | 2.8 |
| T275 (OG1) | D519 (OD1) | 2.9 | T275 (OG1) | T481 (OG1) | 2.9 |
| R282 (NH1) | D525 (OD1) | 3.2 | R282 (NH1) | D525(OD1) | 2.7 |
| R282 (NH1) | D525 (OD2) | 2.6 | R282 (NH1) | D525 (OD2) | 3.1 |
| R282 (NH2) | D525 (OD1) | 2.8 | R282 (NH2) | D525 (OD2) | 2.9 |
| R484 (NH1) | E272 (OE2) | 2.7 | S285 (OG) | F673 (O) | 2.6 |
| R484 (NH2) | E272 (OE2) | 3.2 | D482 (N) | H274 (ND1) | 3.0 |
| R484 (NH2) | Q305 (O) | 2.9 | V483 (N) | G273 (O) | 2.8 |
| R490 (NH1) | E341 (OE1) | 2.8 | R484 (NH1) | E272 (O) | 2.9 |
| R490 (NH1) | E341 (OE2) | 3.3 | R484 (NH1) | E272 (OE1) | 2.8 |
| R490 (NH2) | E341 (OE2) | 2.9 | R489 (NE) | E272 (OE2) | 2.9 |
| K493 (N) | F38 (O) | 2.9 | R489 (NH2) | E272 (OE2) | 2.8 |
| K493 (NZ) | D318 (OD1) | 2.8 | K493 (N) | F38 (O) | 3.1 |
| K493 (NZ) | E321 (OE1) | 2.7 | K493 (NZ) | D318 (OD1) | 3.5 |
| K493 (NZ) | E321 (OE2) | 3.3 | K493 (NZ) | D318 (OD2) | 2.9 |
| K512 (NZ) | E354 (OE1) | 2.8 | K493 (NZ) | E321 (OE2) | 2.8 |
| Q645 (N) | S314 (OG) | 3.3 | S674 (OG) | E287 (OE2) | 2.7 |
| Q645 (NE2) | S313 (O) | 3.5 | |||
| S674 (OG) | E287 (OE1) | 2.6 | |||
| A1:A3 interactions | |||||
| R226 (NH1) | D1909 (OD2) | 3.0 | R220 (NH1) | E1914 (OE2) | 2.8 |
| R226 (NH2) | D1909 (OD1) | 2.9 | R220 (NH2) | E1914 (OE1) | 2.8 |
| R226 (NH2) | D1909 (OD2) | 2.9 | R220 (NH2) | E1914 (OE2) | 3.3 |
| R1719 (NH1) | D221 (OD2) | 2.9 | R226 (NH1) | D1909 (OD1) | 2.9 |
| R1719 (NH2) | D221 (OD1) | 2.8 | R226 (NH1) | D1909 (OD2) | 3.2 |
| R1997 (NH1) | E113 (O) | 2.9 | R226 (NH2) | D1909 (OD2) | 2.7 |
| R1997 (NH2) | E113 (O) | 2.9 | T263 (OG1) | E2004 (OE2) | 2.7 |
| K1972 (NZ) | D150 (OD1) | 2.9 | R1719 (NH1) | D221 (OD1) | 3.1 |
| K1972 (NZ) | D150 (OD2) | 2.9 | R1719 (NH1) | D221 (OD2) | 2.7 |
| N1977 (ND2) | S289 (OG) | 2.9 | R1719 (NH2) | D221 (OD1) | 2.7 |
| N1977 (ND2) | P290 (O) | 2.8 | R1997 (NH1) | E113 (O) | 3.2 |
| K1992 (NZ ) | Y105 (O) | 2.9 | R1997 (NH2) | E113 (O) | 2.8 |
| N1977 (ND2) | P290 (O) | ||||
| K1992 (NZ) | Y105 (O) | 2.8 | |||
| A2:A3 interactions | |||||
| Y664 (N) | K1967 (O) | 3.0 | N414 (ND2) | Q1906 (OE1) | 2.9 |
| T669 (OG1) | P1980 (O) | 3.1 | Y664 (N) | K1967 (O) | 2.9 |
| S695 (OG) | D1842 (OD1) | 2.9 | S695 (OG) | D1842 (OD1) | 2.8 |
| Y729 (N) | E1794 (OE1) | 2.8 | S695 (OG) | D1842 (OD2) | 3.5 |
| Y729 (OH) | R1797 (O) | 2.9 | S732 (OG) | E1794 (OE2) | 2.7 |
| N734 (ND2) | E1793 (OE2) | 2.7 | K733 (NZ) | D1795 (OD1) | 2.7 |
| N735 (ND2) | D1795 (OD1) | 2.8 | R1803 (NH1) | P739 (O) | 2.8 |
| E738 (N) | E1671 (O) | 3.0 | R1803 (NH2) | E738 (OE2) | 2.9 |
| R740 (NH1) | E1811 (OE1) | 2.8 | R1803 (NH2) | P739 (O) | 2.9 |
| R740 (NH2) | E738 (OE1) | 2.7 | K1804 (NZ) | E738 (OE1) | 2.7 |
| R740 (NH2) | E738 (OE2) | 3.4 | K1833 (NZ) | Y664 (O) | 2.8 |
| S1669 (N) | E738 (O) | 3.1 | K1833 (NZ) | D666 (OD1) | 2.8 |
| S1669 (OG) | E738 (OE2) | 2.7 | N1904 (ND2) | N414 (O) | 2.9 |
| V 1670 (N) | P739 (O) | 3.2 | N1904 (ND2) | N414 (OD1) | 3.0 |
| E 1671 (N) | E738 (O) | 3.1 | Q1906 (N) | P606 (O) | 3.0 |
| D 1795 (N) | N734 (OD1) | 3.1 | K1967 (NZ) | E665 (OE1) | 2.9 |
| R 1797 (NH1) | L687 (O) | 3.0 | |||
| T 1826 (N) | Y664 (OH) | 3.1 | |||
| K 1833 (NZ) | Y664 (O) | 2.9 | |||
| K 1833 (NZ) | D666 (OD1) | 2.6 | |||
| R 1900 (N) | E607 (OE1) | 2.7 | |||
| A 1901 (N) | E607 (OE1) | 3.2 | |||
| Q 1906 (N) | P606 (O) | 2.8 | |||
| Q 1906 (NE2) | E604 (OE1) | 2.9 | |||
| K 1967 (NZ) | E665 (OE1) | 2.8 | |||