Figure 6. Functional involvement of the muscarinic M₂ receptor in the TC-induced calcium desynchronization.

(a) Overview of a cluster loaded with 5 µg/ml Fluo-4 AM (scale bar  = 10 µm). Representative of intracellular Calcium dynamics recorded in NRCM pre and post-treatment with 0.2 mmol/L TC. (b) Ca ²⁺ transients recorded in untreated (left panel) and treated (right panel) of control cells. (c) Ca ²⁺ transients recorded in untreated (left panel) and treated (right panel) of scramble (non-targeting) siRNA knockdown cells. (d) Ca ²⁺ transients recorded in untreated (left panel) and treated (right panel) of siRNA M₂ knockdown cells. n>5 observations.