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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Jan 6;48(4):735–745. doi: 10.1016/j.yjmcc.2009.12.022

Figure 6.

Figure 6

DHPR-α2 enrichment increased the percent of cTnT+ cells in LS differentiating media: A. RT-PCR analysis for gene expression evaluation of DHPR-α2, cTnT, cTnI, GATA-4, and GAPDH of IP cells differentiated after day 5, day 15, day 20 and day 26 respectively. An adult heart was used for positive control. B. DHPR-α2 staining of CD117/Sca-1+ enriched cells after 20 days in differentiating media. C. DHPR-α2 flow cytometry analysis of CD117/Sca-1+ enriched cells after 26 days in differentiating media. D. Representative immunofluorescent image of cTnT staining of IP CD117/Sca-1 and DHPR-α2 enriched cells. E. Mean cTnT positive cells 30 days after DHPR-α2 enrichment (n=45) compared to control (n=46) and CD117/Sca-1+ (n=33) cells. Values represent Mean + SEM, and significant differences (*) are P<0.05.