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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Neurobiol Dis. 2010 Jan 4;38(1):17–26. doi: 10.1016/j.nbd.2009.12.021

Figure 3.

Figure 3

Bcl-2 and bcl-w are target genes of miR-497 in mouse N2A cells. The protein levels of bcl-2 and bcl-w are significantly decreased after OGD. This decrease in expression is evident at the 4h recovery time point after 3h of OGD and persisted up to the 24h recovery time point after OGD (A). Treatment of mouse N2A cells with a miR-497 inhibitor effectively increases both bcl-2 and bcl-w levels after OGD (B). In contrast, a miR-497 mimic reduces the protein expression of bcl-2 and bcl-w after OGD (C). The expression of bcl-xl, one bcl-2 family protein, is not affected in mouse N2A cells after treatment with either a miR-497 mimic or inhibitor. The miR-497 mimic and inhibitor controls have no effect on the expression of bcl-2 and bcl-w. Data shown in top panels of figure 3A, B, C are representative of 3 separate experiments with similar results. Quantitative analysis of data in bottom panels of Fig 3A, B, C is expressed as mean ± SD. *p < 0.05 versus non-OGD controls or mimic and inhibitor controls.