Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Neurobiol Dis. 2010 Jan 4;38(1):17–26. doi: 10.1016/j.nbd.2009.12.021

Figure 5.

Figure 5

Bcl-2 and bcl-w are miR-497 target genes in mouse cerebral cortex after transient focal cerebral ischemia. Bcl-2 and bcl-w are downregulated following 24 h-reperfusion after 1h MCAo (A). Mini-pump assisted-cerebroventricular infusion of a miR-497 antagomir effectively inhibits cerebral miR-497 expression (B) and significantly enhances bcl-2 and bcl-w protein levels (C) in mouse cerebral cortex at the 24 h reperfusion time point after 1h MCA occlusion. In contrast, administration of an eGFP mRNA antagomir or artificial CSF (aCSF) has no effect on the expression of miR-497 and its two target proteins. Of note, the expression levels of bcl-xl, another member of the bcl-2 family is not affected in the ischemic cerebral cortex after treatment with the miR-497 antagomir. Quantitative analysis of data with representative blots shown in Fig 5A, C are in the respective bottom panels. Data are expressed as mean ± SD from 3 separate experiments. * denotes p < 0.05 versus sham or eGFP antagomir group.