Figure 7.

Relative quantification of Gmhsp176-L mRNA expression levels (x), in parental PI595099 and BRS133, in inoculated and non-inoculated treatments. Data were obtained by real-time PCR with primers SoyHsp PSC-F and SoyHsp PSC-R, designed using Primer Express Software v20 package and the entire Gmhsp176-L gene sequence available in Genbank (Accession no. M11317). All analyses were carried out using SDS software package. Analyses were done using resistant parental genotype PI595099 as calibrator (value 1X).