Skip to main content
. Author manuscript; available in PMC: 2010 Mar 14.
Published in final edited form as: Genesis. 2008 Dec;46(12):738–742. doi: 10.1002/dvg.20437

FIG. 2.

FIG. 2

Initial Stra8-cre activity is detected in a subset of undifferentiated spermatogonia and its expression is observed through pre-leptotene stage spermatocytes. (a) Cross-section of a Stage VII seminiferous tubule isolated from a 6-week-old Stra8-cre mouse. Arrowheads point to pre-leptotene spermatocytes that express Stra8-cre, detected by anti-cre antibody. (b-c″) Whole-mount immunostaining of seminiferous tubules isolated from two different 2.5-week-old Stra8-cre;Z/EG mice, offspring resulting from a Stra8-cre male × Z/EG female cross. Arrowheads denote undifferentiated spermatogonia expressing the transcriptional repressor PLZF (red) that also express EGFP (green), a result of the excision of the Bgeo gene from the Z/EG allele by Stra8-cre (Merge, yellow). Arrows in b″ and c″ point to PLZF+ spermatogonia that do not express EGFP.