Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Pain. 2010 Feb 18;149(1):100–106. doi: 10.1016/j.pain.2010.01.015

Fig. 5. MK-801 blocks nitration and enzymatic inactivation of MnSOD.

Fig. 5

When compared to the vehicle group (Veh, n=6), injection of carrageenan (Car, n=6) led to significant nitration of MnSOD (A). Co-administration of morphine with MK-801 (2 mg/kg, n=6) prevented the nitration of MnSOD (A). Post-translational nitration of MnSOD (A) led to functional enzymatic inactivation as evidenced by loss of its catalytic activity to dismute superoxide as measured spectrophotometrically (B). MK-801 (2 mg/kg, i.p. n=6) restored the enzymatic activity of MnSOD (B). When compared to the vehicle group, injection of carrageenan did not change the total amount of MnSOD (C) in dorsal horn tissues as measured by Western blotting analysis. All gels shown are representative from gels obtained in 6 animals. The composite mean ± SEM of the densitometry data resulting from n=6 animals is shown in the result section. *P<0.05 for carrageenan vs vehicle; †P<0.01 for carrageenan+MK-801 vs carrageenan alone.