Figure 5.

Norepinephrine activates a Hes5-expressing precursor population. A, Hes5-GFP- positive and -negative cells were sorted using flow cytometry based on their GFP expression. FSC, Forward scatter. B, Reverse transcriptase-PCR analysis revealed the presence of Hes5 mRNA only in the GFP-positive population. C, The Hes5-GFP-positive population contained all the neurosphere-forming cells. Note that in the presence of norepinephrine almost twice as many neurospheres were obtained from the Hes5-GFP-positive population. No neurospheres were generated from the Hes5-GFP-negative population (mean ± SEM; ***p < 0.001).