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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Fungal Genet Biol. 2009 Nov 26;47(3):191–198. doi: 10.1016/j.fgb.2009.11.007

Figure 4.

Figure 4

Evaluation of the deletion of CAP59 and removal of G418 marker. A. To induce capsule formation the strains were grown three days on low iron medium, DME, at 30°C in dark. Strains were stained with 1:1 India Ink:H2O and observed through an Olympus AHBT3 microscope at 1000X magnification. 1. KN99α, production of capsule surrounding the cell wall. 2. cap59Δ (negative control strain), upon induction no discernible capsule is produced and cells clump. 3. lac1ΔG418S, production of capsule surrounding the cell wall is unaffected by the deletion of LAC1. 4. lac1ΔG418Scap59ΔG418R, no discernible capsule is produced and cells clump similar to negative control. B. PCR products using CAP59 specific primers #7 and #8 for 1. wild type CAP59, 2. lac1ΔG418Scap59ΔG418R, and 3. lac1ΔG418Scap59ΔG418S. Molecular weights listed to the left side of gel image.