Skip to main content
. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Biochem Funct. 2009 Jul;27(5):296–308. doi: 10.1002/cbf.1573

Figure 3. CTLs mediated lipid-dependent cytotoxicity in 8 hour 51Cr-release assays only when they were induced with an exceptional lot of IL-4.

Figure 3

Perforin−/− CTLs, cultured with different lots of IL-4 at 500 u/ml, were incubated with 51Cr-radio-labeled P815 cells in anti-CD3 redirected assays that were stopped after 8 hours. The CTL assays were performed with or without trilinolein. The standard errors of the means are illustrated but are so small that they often appear to merge with the symbols. (A). CTLs cultured with lot 83653 IL-4 lacked lipid-enhanced cytotoxicity. This lack of lipid dependent activity was standard and reproducible with multiple lots of IL-4. (B). CTLs cultured with lot 60506 IL-4 displayed lipid-enhanced cytotoxicity by 8 hours. This figure illustrates data representative of two experiments with this lot of IL-4.