Table 1.
IL-4 lotb | Mouse strainsc | Lytic units per 106 CTLs | Lipid enhancementf | |
---|---|---|---|---|
IL-4 CTLsd | IL-4 CTLs + lipide | |||
BD: 60506 | Balb/c WT | 17902 | 29574 | 1.7 |
BD: 60506 | Balb/c WT | 20837 | 29250 | 1.4 |
BD: 60506 | Balb/c WT | 17125 | 23825 | 1.4 |
BD: 60506 | Balb/c perforin KO | 24041 | 74989 | 3.1 |
BD: 60506 | Balb/c perforin KO | 10955 | 38416 | 3.5 |
BD: 60506 | Balb/c perforin KO | 2359 | 11936 | 5.1 |
BD:83653 | Balb/c WT | 11915 | 9402 | 0.8 |
BD:83653 | Balb/c WT | 22222 | 25763 | 1.2 |
BD:83653 | Balb/c perforin KO | 28529 | 29749 | 1.0 |
BD:83653 | Balb/c perforin KO | 23378 | 27227 | 1.2 |
eBio:E028417 | Balb/c WT | 9824 | 8734 | 0.9 |
eBio:E028417 | Balb/c perforin KO | 23820 | 23433 | 1.0 |
eBio:E031047 | Balb/c WT | 35815 | 21322 | 0.6 |
eBio:E031047 | Balb/c perforin KO | 30388 | 37775 | 1.2 |
eBio:E031047 | C57B6 | 35815 | 21322 | 0.6 |
eBio:E031047 | C57B6 perforin KO | 15226 | 11749 | 0.8 |
Cells were cultured for 5 days with 500 units/ml of different lots of IL-4.
The vendors and lots of IL-4. BD, BD Biosciences; eBio, eBioscience.
Mouse strains and genetic modifications.
Lytic units per 10M CTLs for IL-4 induced CTLs without triglycerides.
Lytic units per 10M CTLs for IL-4 induced CTLs with triglycerides.
Ratios of the lytic activity of CTLs assayed in the presence of lipid compared to CTLs without lipid.