. Author manuscript; available in PMC: 2010 Jul 1.

Published in final edited form as: Cell Biochem Funct. 2009 Jul;27(5):296–308. doi: 10.1002/cbf.1573

Table 2.

Lipid-dependent cytotoxicity following alternative methods of CTL induction

IL-4 lot^a	Conditions^b	Activation^c	Mouse strain^d	Lytic units per 10⁶CTLs		Lipid enhancement^g
IL-4 lot^a	Conditions^b	Activation^c	Mouse strain^d	IL-4 CTLs^e	IL-4 CTLs + lipid^f	Lipid enhancement^g
BD:83653	1500 U/ml IL-4	ConA	Balb/c WT	16031	16950	1.1
eBio:E031047	1500 U/ml IL-4	ConA	Balb/c perforin KO	23246	31979	1.4
eBio:E031047	500 U/ml IL-4	ConA	IFNg receptor KO B6	26246	16407	0.6
eBio:E031047	500 U/ml IL-4 + anti-IL-12	ConA	IFNg receptor KO B6	46398	16408	0.4
eBio:E031047	500 U/ml IL-4	Anti-CD3/CD28	C57B6	30063	27780	0.9
eBio:E031047	500 U/ml IL-4	Anti-CD3/CD28	C57B6	23088	27931	1.2
eBio:E031047	500U/ml IL-4 + 400U/ml IFNg	ConA	C57B6	3543	1351	0.4

Lot of IL-4.

Concentrations of cytokine during induction of T cells.

Method of T cell activation.

Mouse strain and genetic modifications.

Lytic units per 10M CTLs for IL-4 induced CTLs assayed without triglycerides.

Lytic units per 10M CTLs for IL-4 induced CTLs assayed with triglycerides.

Ratios of the lytic activity of CTLs assayed in the presence of lipid compared to CTLs without lipid.