FIG. 4.

Relative expression ratio of cytokines in spleen of vaccinated chickens compared to that of control birds. The expression of IFN-γ (A) and IL-10 (B) was compared between chickens vaccinated with TROVAC (n = 6) and control chickens that were treated with vaccine diluent only (n = 6). Target and reference gene expression in spleen cells was quantified by real-time RT-PCR, and expression ratios were calculated as the means of gene expression in samples of vaccinated group compared to that of controls. An asterisk indicates significant difference (P < 0.05). Error bars represent standard errors from the means.