FIG. 5.

In vitro proliferative responses of splenocytes to recombinant HA antigen. Chickens were vaccinated with TROVAC-AIV H5 or received vaccine diluent as a control. Splenocytes from four vaccinated chickens were treated with HA or medium and are shown as Vac. HA and Vac. medium, respectively. Also, splenocytes from four unvaccinated chickens (as controls) were treated with HA and medium and are shown as Cont. HA and Cont. medium. The differences in proliferative responses among the groups at each time point were tested and considered significant at P ≤ 0.05 (*).