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Cross-reactivity of sera against PspA. Sera from four BALB/c mice immunized with PspA1, PspA2, PspA3, PspA4, or PspA5 were collected after the second immunization and analyzed individually by ELISA against each PspA fragment. The results are shown as the log10 of the titer. An asterisk indicates a statistically significant difference from animals immunized with alum (Student's t test, P ≤ 0.05). Results are representative of two experiments using sera from independent immunizations.
