FIG. 5.

Inflammatory profile induced by C16:0-LPC and C18:0-LPC. Sera from anesthetized C57BL/6 mice receiving intravenous injections of either C16:0-LPC (500 nmol), C18:0-LPC (500 nmol), CpG-ODN1826 (10 μg and 25 μg), LPS (10 μg and 25 μg), or poly(I:C) (10 μg and 25 μg) were assayed for the presence of cytokines by using a Milliplex map kit. The data for two mice are included per time point; and sera were collected at 2 h (mouse 1 [M1], M2), 4 h (M3, M4), or 12 h (M5, M6). (A) IL-6. The positive thresholds, defined as two times the mean level of secretion for PBS-injected mice, were 45 pg/ml at 2 h, 45 pg/ml at 4 h, and 0 pg/ml at 12 h. (B) IL-5. The positive thresholds were 61 pg/ml at 2 h, 78 pg/ml at 4 h, and 39 pg/ml at 12 h.