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. 2010 Mar;9(3):424–437. doi: 10.1128/EC.00348-09

Fig. 3.

Fig. 3.

Functional analysis of zrfC in the S. cerevisiae background. The yeast strain ZHY3 was transformed with derivative pRS316 plasmids carrying, under the control of the ZRT1 promoter, the coding sequence of ZRT1 (pMC5-HSET), zrfA (pZHA1), zrfB (pZHA2), zrfC (pZSF30), or zrfCΔ13→622 (zrfCΔN) (pZSF310), as indicated on the left side of each picture. The DY1457 strain transformed with pRS316 is formally considered to be a wild type (wt). A total of 104 yeast cells were spotted onto SDA zinc-limiting agar plates buffered at pH 4.4 (A) or 7.5 (B) with 100 mM potassium phosphate and supplemented with Zn2+ at the specified concentrations (0 to 2,000 μM). Acid plates were supplemented with 1 mM EDTA and incubated for 2 days at 28°C. Alkaline plates were supplemented with 0.1 mM EDTA and incubated for 2 and 4 days at 28°C.