Fig. 5.

Construction and phenotypic analyses of AF2511-derivative pyrG⁺ strains of A. fumigatus. (A) Construction of A. fumigatus strains that express zrfA (AF751), zrfB (AF761), zrfC (AF731), or zrfC^Δ13→622 (zrfC^ΔN; AF791) at the pyrG locus. All strains harbored the correct integration event, as verified by Southern blotting analyses (not shown). The dashed boxes represent the terminator of the aspnd1 gene, as described in Materials and Methods. (B) Growth of the same strains on both acid (SDAE, pH 4.5) and alkaline (SDNE, pH 7.5) zinc-limiting agar media not supplemented with Zn²⁺ or supplemented with 1 to 1,000 μM Zn²⁺, as indicated at the top of each panel.