FIG. 5.
CaDPA release and ELS intensities for six individual B. cereus spores during wet-heat treatment at 80°C. (a) Relative intensities of the CaDPA-specific Raman band at 1,017 cm−1 for six individual spores (spores a to f) were determined as described in Materials and Methods. (b) Relative ELS intensities of the six spores described in the legend for panel a were determined as described in Materials and Methods. The baselines of ELS intensity were shifted for display, and the dashed line below each ELS signal is the corresponding zero level. The downward and upward arrows mark Tlag and Trelease, respectively, for each spore.