TABLE 1.
Mean values and standard deviations of Tlag, Trelease, ΔTrelease, T1, and T2 and mean CaDPA levels at T1 of 20 individual spores incubated at high temperatures in watera
| Spore and temperature | Tlag (min) | Trelease (min) | ΔTrelease (min) | T1 (min) | CaDPA level at T1 (%) | T2 (min) |
|---|---|---|---|---|---|---|
| B. megaterium at 80°C | 6.1 ± 3.4 | 7.1 ± 3.3 | 1.0 ± 0.3 | 6.8 ± 3.4 | 24.0 | 7.5 ± 3.3 |
| B. cereus at 80°C | 7.7 ± 4.1 | 8.8 ± 4.1 | 1.1 ± 0.2 | 8.6 ± 4.1 | 20.0 | 9.7 ± 4.3 |
| PS832 at 90°C | 11.2 ± 5.6 | 12.6 ± 6.4 | 1.4 ± 1.0 | 11.6 ± 5.7 | 53.0 | 12.8 ± 6.2 |
| PS533 at 90°C | 14.4 ± 6.8 | 16.3 ± 7.9 | 2.0 ± 1.4 | 14.9 ± 7.0 | 41.0 | 16.5 ± 8.1 |
a
Spores of various Bacillus species or strains were incubated in water at various temperatures, and Raman spectra and ELS intensities for 20 individual spores in a dual trap as well as various parameters were determined as described in Materials and Methods and other portions of the text.