TABLE 1.
Oligonucleotide primers for multiplex PCR and sequencing
| Assay | Target gene or primer name | Forward primer | Reverse primer | Product size (bp) | Reference |
|---|---|---|---|---|---|
| Assay 1 | stx1 | GTGGCATTAATACTGAATTGTCATCA | GCGTAATCCCACGGACTCTTC | 109 | 29 |
| stx2 | GGCACTGTCTGAAACTGCTCC | TCGCCAGTTATCTGACATTCTG | 255 | 47 | |
| Assay 2 | stI | TCTTTCCCCTCTTTTAGTCAGTC | CAGCACAGGCAGGATTAC | 170 | 36 |
| elt | ACGGCGTTACTATCCTCTC | TGGTCTCGGTCAGATATGTG | 274 | 48 | |
| daaE | GAACGTTGGTTAATGTGGGGTAA | TATTCACCGGTCGGTTATCAGT | 542 | 54 | |
| aafII | CACAGGCAACTGAAATAAGTCTGG | ATTCCCATGATGTCAAGCACTTC | 378 | 54 | |
| Assay 3 | eae | CTGAACCAGATCGTAACGGC | TGATAAGCTGCAGTCGAATCC | 229 | 36 |
| bfp | AACCGTTACTGCCGGTGTGA | GTTGCCGCCTCAGCAGGAGT | 450 | 36 | |
| ipaH | CTCGGCACGTTTTAATAGTCTGG | GTGGAGAGCTGAAGTTTCTCTGC | 933 | 54 | |
| Assay 4 | eae | CTGAACCAGATCGTAACGGC | TGATAAGCTGCAGTCGAATCC | 229 | 36 |
| EHEC hlyA | AGCCGGAACAGTTCTCTCAG | CCAGCATAACAGCCGATGT | 526 | 35 | |
| stx1 sequencing | Paton 1 | TCGCATGAGATCTGACC | AACTGACTGAATTGAGATG | 1,470 | 5 |
| Paton 2 | ATAAATCGCCATTCGTTGACTAC | AGAACGCCCACTGAGATCATC | 180 | 5 | |
| Gannon | ACACTGGATGATCTCAGTGG | CTGAATCCCCCTCCATTATG | 603 | 5 | |
| Vidiya | TCGCATGAGATCTGACC | AATAAGCCGTAGATTATT | 448 | 5 | |
| stx2 sequencing | Stx2-1 | TTCTGAGCAATCGGTCACTG | CGGCGTCATCGTATACACAG | 779 | 55 |
| Stx2-2 | GTCACAGCAGAAGCCTTACG | ACCCACATACCACGAATCAG | 714 | 55 |