FIG. 1.

(Left panel) Outer membrane profiles of the ΔalgE mutant and the ΔalgE mutant harboring various plasmids showing the absence or presence of AlgE. Faint bands with the same apparent molecular mass (indicated on the left, in thousands) as AlgE present in the PDO300ΔalgE(pBBR1MCS-5) and PDO300ΔalgE(pBBR1MCS-5:algEΔL7) outer membrane profiles were identified as flagellin type B protein by MALDI-TOF MS. (Right panels) Presence of tagged AlgE proteins in the outer membrane, as indicated by Western immunoblotting (middle). Constitutively produced OprF was used to standardize the samples (bottom).