Strains |
|
|
P. aeruginosa
|
|
|
PDO300 |
mucA22 isogenic mutant derived from PAO1 |
18 |
PDO300ΔalgE
|
Isogenic algE deletion mutant derived from PDO300 |
This study |
E. coli
|
|
|
TOP10 |
E. coli cloning strain |
Invitrogen |
S17-1 |
thi-1 proA hsdR17 (rK− mK+) recA1; tra gene of plasmid RP4 integrated in chromosome |
39 |
Plasmids |
|
|
pGEM-T Easy |
Apr, Plac
|
Invitrogen |
pBBR1MCS-5 |
Gmr; broad-host-range vector; Plac
|
23 |
pBBR1MCS-5:algE
|
HindIII-BamHI fragment comprising algE inserted into vector pBBR1MCS-5 |
This study |
pBBR1MCS-5:algEDelL7 |
algE fragment with 57-bp deletion in region encoding putative surface exposed loop 7 inserted into pBBR1MCS-5 |
This study |
pBBRMCS-5:algEL1FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 189th bp of the ORF |
This study |
pBBRMCS-5:algEL2FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 336th bp of the ORF |
This study |
pBBRMCS-5:algEL3FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 468th bp of the ORF |
This study |
pBBRMCS-5:algEL4FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 582nd bp of the ORF |
This study |
pBBRMCS-5:algEL5FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 711th bp of the ORF |
This study |
pBBRMCS-5:algEL6FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 882nd bp of the ORF |
This study |
pBBRMCS-5:algEL7FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 1035th bp of the ORF |
This study |
pBBRMCS-5:algEL8FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 1269th bp of the ORF |
This study |
pBBRMCS-5:algEL9FLAG |
algE fragment with a 24-bp insertion (encoding the FLAG epitope) inserted after the 1428th bp of the ORF |
This study |
pEX100T |
Apr Cbr; gene replacement vector containing sacB gene for counterselection |
17 |
pEX100T:ΔalgEGm |
Apr Cbr Gmr; vector pEX100T with SmaI-inserted algE deletion construct |
This study |
pPS865 |
Apr Gmr; source of 1,100-bp BamHI fragment comprising aacC1 gene flanked by Flp recombinase target site signal sequences |
17 |
pPFLP2 |
Apr Cbr; broad-host-range vector encoding Flp recombinase |
17 |