TABLE 2.
Production of alginate and free uronic acids by ΔalgE mutants
| Strain | Mean alginate concn (g/g CDWa) ± SD | Free uronic acids |
|
|---|---|---|---|
| Sourceb | Mean concn (g/g CDW) ± SD | ||
| PDO300(pBBR1MCS-5) | 0.216 ± 0.027 | Total | 0.524 ± 0.076 |
| Filtrate | 0.044 ± 0.009 | ||
| PDO300ΔalgE(pBBR1MCS-5) | NDc | Total | 0.486 ± 0.050 |
| Filtrate | 0.425 ± 0.039 | ||
| PDO300ΔalgE(pBBR1MCS-5:algE) | 1.096 ± 0.251 | Total | 0.500 ± 0.014 |
| Filtrate | 0.268 ± 0.010 | ||
| PDO300ΔalgE(pBBR1MCS-5:algEΔL7) | ND | Total | 0.249 ± 0.007 |
| Filtrate | 0.261 ± 0.031 | ||
| PDO300ΔalgX(pBBR1MCS-5) | ND | Total | 0.138 ± 0.002 |
| Filtrate | 0.131 ± 0.001 | ||
| PDO300Δalg8(pBBR1MCS-5) | ND | Total | 0.012 ± 0.002 |
| Filtrate | 0.013 ± 0.001 | ||
a
CDW, cellular dry weight.
b
Total, concentration of uronic acids in the unfiltered culture media (including alginate and free uronic acids); Filtrate, concentration of uronic acids in the filtered culture media (free uronic acids).
c
ND, none detected.