TABLE 1.
PCR primer sequences used in this study
| Target | Sequence (5′-3′) | Target positiona | Annealing temp (°C) | Extension time (s) | Reference |
|---|---|---|---|---|---|
| Group U2 | FW, CTAGGTGTAGGGGGTATC | 834 | 60 | 18 | This study |
| RV, GCTGCCCTCTGTCGTTG | 1259 | ||||
| Group U3 | FW, TTTGAAACTGTCTAGCTAGAGTA | 64 | 64 | 26 | This study |
| RV, CATCACTGCTCTGCTTCC | 1253 | ||||
| F. succinogenes | FW, GGTATGGGATGAGCTTGC | 219 | 60 | 18 | Koike et al. (13) |
| RV, GCCTGCCCCTGAACTATC | 654 | ||||
| Total bacteria | FW, CCTACGGGAGGCAGCAG | 341 | 60 | 10 | Koike et al. (13) |
| RV, ATTACCGCGGCTGCTGG | 534 |
a
The positions are based on the nucleotide position in Escherichia coli.