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Real-time PCR quantification of group U2 and total bacteria during the enrichment processa
| Sample source | Group U2 (×108 copies/g) | Total (×1011 copies/g) | % of total |
|---|---|---|---|
| Rumen digesta | 4.82 ± 0.08 | 1.34 ± 0.11 | 0.36 ± 0.03 A |
| Inoculum | 34.20 ± 0.59 | 3.07 ± 0.25 | 1.12 ± 0.10 B |
| 1-day culture | 7.55 ± 0.13 | 0.50 ± 0.04 | 1.53 ± 0.12 C |
| 5-day culture | 9.78 ± 0.17 | 0.33 ± 0.03 | 2.95 ± 0.19 D |
Within a column, means followed by different capital letters differed significantly (P < 0.05).
