TABLE 1.
Information relevant to the nine-gene MLST loci new to this study
| Locus | Size (bp) | No. of alleles | % Variable sites | dN/dS ratioa | Coordinatesb | Primer sequence (5′-3′) |
|
|---|---|---|---|---|---|---|---|
| Forward | Reverse | ||||||
| adk | 471 | 7 | 1.49 | 1.0086 | 599978-600448 | TAATTATAGGTGCACCAGGAAGCG | GGTTCGATAGTGCGTTCTCCATC |
| aroE | 675 | 7 | 3.26 | 0.0809 | 369933-370607 | ACAATGCCATTCAAGCTCTCAAGC | CAGAGCTTCTTGCATGGCATTT |
| mdh | 630 | 20 | 14.29 | 0.2716 | 1096816-1097445 | GGGTTTTGTGGGTGCAGCAA | CAAATTCGGTTCTTCCTTTGCG |
| nadP | 606 | 28 | 7.43 | 0.1061 | 1478490-1479096 | AATGGTCGCATTCAAAGCAAGG | ACGCCCATAGCCAAAGCATC |
| pheS | 594 | 11 | 2.53 | 0.096 | 839250-838653 | AGATTTAGCGGGCGAAGAAAAG | CACAACCCTTGCAAAATACACACG |
| pgi | 594 | 18 | 2.19 | 0.7806 | 1466697-1467290 | TGGTTCAAGTTGCGGTGTTAAAGC | TGCTGATCCCTTGCTCCTATAAGAG |
| recA | 615 | 11 | 13.33 | 0.0234 | 1594041-1594655 | CAGGTTCTGTAGGACTTGATCTTGC | TCAGCTTGTCTAAATGGAGGTGC |
| carB | 603 | 9 | 9.29 | 0.0283 | 642616-643218 | TTGGCACTTGATTTAACCGCAG | GCAGCGTCAAGATCAGGAAAGATA |
| trmU | 561 | 25 | 10.87 | 0.0276 | 68933-69497 | TTCTAGTCGCTATGAGCGGTGG | CGTGTTTTCCTACAACCTTACCGCT |
a
Ratio of nonsynonymous to synonymous evolutionary changes.
b
The genomic coordinates are relative to those of the Campylobacter jejuni NCTC 11168 genome.