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Analysis of phage Φ1-A4 structural proteins. After the purification of Φ1-A4 by CsCl density gradient centrifugation, the phage-containing band was analyzed by 4 to 12% SDS-PAGE. Lane M, molecular mass marker; lane Φ, proteins from Φ1-A4. The N-terminal amino acid sequences of the five structural proteins were determined. The position of the N-terminal sequence in the corresponding Φ1-A4 ORF and its proposed function are indicated.
