TABLE 1.

Primers used in the study^a

Primer	Sequence	Binding site	Amplicon size (bp)	Melting peak (°C)
TPI A forward primer	5′-TCGTCATTGCCCCTTCCGCC-3′	116-135	77	86
TPI B sequence	5′-.T..TG....T..C...TTT-3′
TPI A reverse primer	3′-CAGTTGAGGATAGCAGCG-5′	175-192
TPI B sequence	3′-TGTC...AA.........-5′
TPI B forward primer	5′-GATGAACGCAAGGCCAATAA-3′	397-416	77	80
TPI A sequence	5′-..C..G...........CCG-3′
TPI B reverse primer	3′-AAGAAGGAGATTGGAGAATC-5′	454-473
TPI A sequence	3′-GGC......C.C.....G..-5′
GDH A forward primer	5′-CCGGCAACGTTGCCCAGTTT-3′	710-729	180	83
GDH B sequence	5′-.T...........T....AC-3′
GDH A reverse primer	3′-TCCGAGTTCAAGGACAAGT-5′	871-889
GDH B sequence	3′-G.T...........G....-5′
GDH B forward primer	5′-CGTATTGGCGTCGGCGGT-3′	492-510	133	83
GDH A sequence	5′-...C..C..........CC-3′
GDH B reverse primer	3′-CTATCAGACCAGAGGCCACA-5′	604-623
GDH A sequence	3′-T..........G........G-5′
ORFC4 A forward primer	5′-CTGTAGACAGGGCCCAGGCC-3′	135-154	103	85
ORFC4 B sequence	5′-......G....AG..G.ATG-3′
ORFC4 A reverse primer	3′-ATTAAGGGCAGGGGACATCAT-5′	217-237
ORFC4 B sequence	3′-GGC...T.T.A.....G....-5′
ORFC4 B forward primer	5′-ACTGTCCATTTCTATCTGAG-3′	281-300	171	79
ORFC4 A sequence	5′-C...C........G..ATCC-3′
ORFC4 B reverse primer	3′-AGGTGGAGGCCAATGGAATCC-5′	431-451
ORFC4 A sequence	3′-CT..A.....AG........A-5′

^aThe sequence of each assemblage-specific primer is aligned over the corresponding sequence of the other assemblage. The dots indicate identical nucleotides. The 3′ ends of each primer are in boldface. The binding site, amplicon size, and melting peaks are also given.