FIG. 3.

Detection of multiple pathogenic Vibrio species in a sample collected in August 2006 from the Chorokhi estuary site on the coast of the Black Sea. (A) Mass spectra of PCR amplicons derived from the RNase P housekeeping gene from total community DNA extracted from the sample showing detections of V. cholerae, V. parahaemolyticus, and V. vulnificus. (B) Relative proportion of DNA sequences matching Vibrio species obtained from an environmental 16S rRNA clone library using primers 24F and 680R, as described in Materials and Methods. For comparison, the genomic quantifications (numbers of genome copies per reaction mixture) derived from the PCR/ESI-MS assay are given in parentheses.