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. 2010 Jan 29;192(7):1937–1945. doi: 10.1128/JB.01629-09

FIG. 2.

FIG. 2.

Complexes of OxyR or SeqA with agn43 HM-DNA are displaced rapidly by an excess of agn43 HM-DNA. Shown are data from EMSA analysis of 0.2 pmol HM-DNA (a and b) or 0.4 pmol unmethylated agn43 DNA as a comparison (c). Samples have no protein (lanes 1) or an addition of 9 pmol SeqA (a) or 9 pmol OxyR (b and c), and complexes were allowed to form and then incubated for a further 10 min with no addition (lanes 2) or with the addition of non-FAM-labeled agn43 HM-DNA at 0.3 pmol (lanes 3), 0.6 pmol (lanes 4), 1.2 pmol (lanes 5), and 2.8 pmol (c, lane 6).