TABLE 2.
Oligonucleotides used in this work
| Oligonucleotide | Sequence (5′-3′)a | Function |
|---|---|---|
| oMV102 | GGAATTCCCGTCATGTGATTCCATAC | R, agn(+275)b |
| oMV329 | AATGCCTCATGATGTGTAGGCTGGAGCTGCTTC | F, cat/FRT |
| oMV330 | AATTGGCGGCCGCCATGGGAATTAGCCATGGTCC | R, cat/FRT |
| oMV396 | CGCGTCGACTCCTGCACAGGCACAGAAT | F, agn(−466) |
| oMV397 | CGCGTCGACTCTTCTGAACCTGTCGTGAC | F, agn(−112) |
| oMV419 | CATACTAGTCGACTCAATAATAGAATAAAACG | R, agn(−17); OxyR BS |
| oMV420 | GGCAACTCGAGTACTGACAAAGGTTATTAC | F, agn(+64); OxyR BS |
| oMV659 | GTCGACGTCGGCTTGAGAAAGACCTG | F, oriC (nt 3923331)c |
| oMV687 | GTCGACTATTGCACGGACAAGAGAG | R, agn(−39) |
a
Restriction sites are in boldface type.
b
agn sequences are from E. coli K-12 MG1655; the coordinate is 5′ relative to the +1 transcription start site (46). F and R indicate forward and reverse orientations, respectively.
c
According to MG1655 genome numbering.