TABLE 3.
Phase variation of agn43 is biased to the off phase as a result of introducing auxiliary OxyR binding sitesa
| Strain | No. of auxiliary OxyR BS and insertion site | Switch frequency |
|
|---|---|---|---|
| Off to on | On to off | ||
| MV1116 | agn′(−112)-lacZ | 2.3 × 10−3 | 1.9 × 10−3 |
| MV1154 | +1 OxyR BS at −112 | 3.4 × 10−3 | 4.6 × 10−3 |
| MV1276 | +2 OxyR BS at −112 | 5.7 × 10−3 | 3.7 × 10−3 |
| MV1190 | +3 OxyR BS at −112 | 3.4 × 10−3 | 25.2 × 10−3 |
| MV1278 | +4 OxyR BS at −112 | NA | NA |
| MV1115 | agn′(−466)-lacZ | 3.7 × 10−3 | 0.7 × 10−3 |
| MV1153 | +1 OxyR BS at −466 | 2.9 × 10−3 | 2.0 × 10−3 |
| MV1277 | +2 OxyR BS at −466 | 4.3 × 10−3 | 1.1 × 10−3 |
| MV1279 | +3 OxyR BS at −466 | NA | NA |
| MV1311 | Inverted [agn′(−466)-lacZ + 1 OxyR BS] | 4.9 × 10−3 | 0.7 × 10−3 |
| MV1314 | Inverted [agn′(−466)-lacZ + 3 OxyR BS] | 3.7 × 10−3 | 13.8 × 10−3 |
a
Representative data are given.
b NA, not applicable (locked off).