TABLE 1.
Bacterial strains, plasmids, and primers
| Strain, plasmid, or primer | Relevant characteristics | Source and/or reference |
|---|---|---|
| Strains | ||
| PAK | R1-pyocin producer, LPS A+ LPS B+ | J. Lam |
| PAO1 | R2-pyocin producer | Laboratory collection |
| PA14 | R2-pyocin producer | 6 |
| 15108/-1 | R5-pyocin producer, clinical isolate | 11 |
| PAKΔR1 | ΔPA0620-PA0621 in R1-pyocin region | This study |
| PA14ΔR2 | ΔPA0620-PA0621 in R2-pyocin region | This study |
| 15108/-1ΔR5 | ΔPA0620-PA0621 in R5-pyocin region | This study |
| PAKwbpP | LPS A+ LPS B− | J. Lam (1) |
| PAKwbpO | LPS A+ LPS B− | J. Lam (1) |
| PAKwbpL | LPS A− LPS B− | J. Lam (1) |
| PAKrmlC | LPS A− LPS B−l-Rha− | J. Lam (20) |
| PAO1wbpM | LPS A+ LPS B− | J. Lam (2) |
| PAO1wbpL | LPS A− LPS B− | J. Lam (22) |
| PAO1rmlC | LPS A− LPS B−l-Rha− | J. Lam (20) |
| PAO1rmd | LPS A− LPS B+ | J. Lam (22) |
| AK1012 | PAO1algC, LPS A− LPS B−, core deficient | 9 |
| Plasmids | ||
| pEX18T | Suicide vector, Tetr | 8 |
| pRK2013 | RK2, helper plasmid | 4 |
| pΔR2T42 | R2 pyocin region of PA14 harboring a 2,285-bp deletion encompassing PA0620 and PA0621 | This study |
| Primers | ||
| PA0620 R5-F | 5′-CGATACAGGACTCATCGTGCAG-3′ | This study |
| PA0620 R5-R | 5′-ACTCGGTAGCGACAATCACACA-3′ | This study |
| PA0621-L | 5′-CCTCCTTGCGAGAGGTTTATGA-3′ | This study |
| PA0621-R | 5′-ATCTCGATGGCGAACAACTAGG-3′ | This study |
| PA0617-L | 5′-CGGCCTGGCTCATCTTAAACA-3′ | This study |
| PA0617-R | 5′-ACTCGCACGGCAGACAATCC-3′ | This study |
| PA0628-L | 5′-CTTCGACGTTACCTTCGACGAC-3′ | This study |
| PA0628-R | 5′-CTCTCCACGGCTGGTATAGAGG-3′ | This study |
| PA0620 R1-F | 5′-GCGCACATGCAGACCATATCTA-3′ | This study |
| PA0620 R1-R | 5′-GGTATCGTGGTTATGCCCTGAG-3′ | This study |
| PA0619-FD | 5′-CGCTTCGTGAGCTTAACGATC-3′ | This study |
| PA0622-RD | 5′-GCAGGCCAGGTAGATAGACGAG-3′ | This study |