FIG. 3.

Mutations found in the kinase domain-coding region of W83 fimS. (A) Two regions in the kinase domain of W83 FimS differ from that of 33277 FimS. 1, a single-amino-acid substitution from isoleucine to lysine; 2, truncation of the C-terminal region containing the G3 box. (B) Alignments of fimS nucleotide and deduced amino acid sequences around the FimS kinase domain. 1, a missense mutation (ATA [I₅₁₁] to AAA [K₅₁₈]) is located upstream of the N box (NLLSNA) coding region; 2, an insertion of A (indicated by an arrow) is found downstream of the G2 box (GSGLGL) coding region of W83. This insertion caused a frameshift mutation and truncated the C-terminal coding region that contains the predicted G3 box (GKGSVF). The VDD motif in FimR is conserved in the receiver domains of response regulators. (C) Alignments of amino acid sequences around the distinct regions, 1 and 2, of 33277 and W83 FimS (Pg327S and PgW83S, respectively) with histidine kinases from other Bacteroidetes. Bv, Bacteroides vulgatus ATCC 8482; Bt, Bacteroides thetaiotaomicron VPI-5482; and Bf, Bacteroides fragilis NCTC9343. Numbers followed by Bv, Bt, and Bf represent gene locus numbers of histidine kinases in each organism. Note that the predicted G3 box (G+GS+F) in region 2 is highly conserved beyond species, whereas the amino acid indicated by an asterisk in region 1 is not.