FIG. 2.

SDS-PAGE and Western blotting of S. Typhimurium strains. (A) Proteins of the membrane fraction were separated by SDS-PAGE and stained with Coomassie brilliant blue. (B) Western blot analysis using a monoclonal antibody against Synechocystis Δ¹²-desaturase shows that it was present in the membrane fraction of Stm(pΔ¹²). Lanes kDa, protein markers; lanes 1, membrane proteins from Stm(pNir) cells; lanes 2, membrane proteins from Stm(pΔ¹²). The arrow indicates the position of the Δ¹²-desaturase, with an apparent molecular mass of 36 kDa.