TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Escherichia coli BL21(DE3)/pLysS | Overproduction of proteins | Novagen |
| Salmonella strains | ||
| SJW1103 | Wild type for motility and chemotaxis | 31 |
| SJW1364 | flhA | 10 |
| SJW1368 | Δ(cheW-flhD); master operon mutant | 25 |
| SJW2228 | flhA(G368C) | 28 |
| SJW2228iH | flhA(G368C) ΔfliH | This study |
| MMA2228-1 | flhA(G368C/L359F) | This study |
| MMA2228-3 | flhA(G368C/G364R) | This study |
| MMA2228-7 | flhA(G368C/R370S) | This study |
| MMA2228-9 | flhA(G368C/P550S) | This study |
| MMHI001 | ΔfliH ΔfliI | 17 |
| MMHI0117 | ΔfliH ΔfliI flhB(P28T) | 23 |
| MMHI0117-28 | ΔfliH ΔfliI flhB(P28T) flhA(G368C) | This study |
| Plasmids | ||
| pGEX-6p-1 | Expression vector | GE Healthcare |
| pTrc99A | Expression vector | GE Healthcare |
| pTrc99AFF4 | Expression vector | 26 |
| pGKK1702 | pGEX-6p-1/GST-FliI | 8 |
| pMM102 | pTrc99A/His-FlhAC | 21 |
| pMM102(G368C) | pTrc99A/His-FlhAC(G368C) | This study |
| pMM104 | pET19b/His-FlhAC | 21 |
| pMM104(G368C) | pET19b/His-FlhAC(G368C) | This study |
| pMM104(G368C/L359F) | pET19b/His-FlhAC(G368C) | This study |
| pMM104(G368C/G364R) | pET19b/His-FlhAC(G368C/G364R) | This study |
| pMM104(G368C/R370S) | pET19b/His-FlhAC(G368C/R370S) | This study |
| pMM104(G368C/P550S) | pET19b/His-FlhAC(G368C/P550S) | This study |
| pMM130 | pTrc99AFF4/FlhA | 9 |
| pMM130(L359F) | pTrc99AFF4/FlhA(L359F) | This study |
| pMM130(G364R) | pTrc99AFF4/FlhA(G364R) | This study |
| pMM130(R370S) | pTrc99AFF4/FlhA(R370S) | This study |
| pMM130(P550S) | pTrc99AFF4/FlhA(P550S) | This study |
| pMM306 | pTrc99A/His-FliH | 21 |
| pMM310 | pET19b/His-FliH | 21 |
| pMM1702 | pTrc99A/His-FliI | 21 |
| pMM309iI | pTrc99AFF4/FliH+His-FliI | 20 |
| pMMJ1001 | pGEX-6p-1/GST-FliJ | This study |
| pMMHB1001 | pGEX-6p-1/GST-FlhBC | 24 |
| pTIH001 | pGEX-6p-1/GST-FliH | T. Ibuki, unpublished data |